Journal: Nutrients

Article Title: Integrated Proteomics and Metabolomics Reveal the Direct Hepatic Protection of Propionate Against Alcoholic Liver Disease via the RGN-PPARα Pathway

doi: 10.3390/nu18050872

Figure Lengend Snippet: The effect of PA on lipid accumulation in ALD mice. ( A ) Schematic of the acute alcoholic liver injury model in mice with propionate treatment. ( B , C ) Serum ALT and AST levels in mice. ( D , E ) H&E and Oil Red O staining of liver tissues (scale bar: 50 μm). ( F ) The relative area of Oil Red O staining. ( G , H ) Liver levels of TC and TG in mice. ( I – L ) Serum concentrations of TC, TG, HDL-C and LDL-C in mice. ( M ) Representative Western blot analysis and ( N ) quantification of ACC1, SREBP1 and CPT1A protein expression in liver tissues normalized to β-actin (n = 4). The data are presented as means ± SEM. * p < 0.05; ** p < 0.01.

Article Snippet: Primary antibodies, including β-actin (#66009-1-Ig), CPT1A (#15184-1-AP), SREBP-1 (#14088-1-AP), ACC1 (#21923-1-AP), ACOX1 (#83731-2-RR), PPARα (#66826-1-Ig) and RGN (#17947-1-AP), were obtained from Proteintech Group (Wuhan, China).

Techniques: Staining, Western Blot, Expressing

Journal: Nutrients

Article Title: Integrated Proteomics and Metabolomics Reveal the Direct Hepatic Protection of Propionate Against Alcoholic Liver Disease via the RGN-PPARα Pathway

doi: 10.3390/nu18050872

Figure Lengend Snippet: Propionate suppressed cell injury and lipid accumulation in vitro. ( A ) Changes in AML-12 cell viability after a series of doses of ethanol challenge. ( B ) Changes in AML-12 cell viability after a series of concentrations of OA incubation. ( C ) Effects of different concentrations of propionate on the viability of AML-12 cells. ( D ) Propionate restored AML-12 cells’ viability following co-treatment with 200 mM ethanol and 0.5 mmol/L OA. ( E ) Representative views of Oil Red O staining of AML-12 cells (scale bars: 100 μm and 50 μm). ( F ) Representative images of Western blot analysis and ( G ) quantification of ACC1, SREBP1 and CPT1A protein expression in AML-12 cells normalized to β-actin (n = 3). The data are presented as means ± SEM. * p < 0.05; ** p < 0.01.

Article Snippet: Primary antibodies, including β-actin (#66009-1-Ig), CPT1A (#15184-1-AP), SREBP-1 (#14088-1-AP), ACC1 (#21923-1-AP), ACOX1 (#83731-2-RR), PPARα (#66826-1-Ig) and RGN (#17947-1-AP), were obtained from Proteintech Group (Wuhan, China).

Techniques: In Vitro, Incubation, Staining, Western Blot, Expressing

Journal: Molecular medicine (Cambridge, Mass.)

Article Title: PI3K p85α/HIF-1α accelerates the development of pulmonary arterial hypertension by regulating fatty acid uptake and mitophagy.

doi: 10.1186/s10020-024-00975-9

Figure Lengend Snippet: Fig. 1 HIF-1α was closely associated with the Parkin/PINK1 axis and fatty acid metabolism-related factors in PAH patients. A Serum levels of HIF-1α were measured by ELISA. B Serum levels of Parkin and PINK1 were measured by ELISA. C Serum levels of FAS, ACC, and CD36 were measured by ELISA. *P < 0.05 vs. Normal. The statistical test was performed using unpaired Student’s t-test. D Spearman’s correlation analysis was performed to determine the correlation between HIF-1α, Parkin, PINK1, FAS, ACC, and CD36. *P < 0.05. ***P < 0.001. ****P < 0.0001

Article Snippet: The levels of HIF-1α, Parkin, PINK1, fatty acid synthetase (FAS), acetyl-CoA carboxylase (ACC), and CD36 in human or rat serum were measured according to the instructions of the following ELISA kits: Human HIF-1α ELISA kit (CSB-E12112h, CUSABIO Co., Ltd., Wuhan, China), Human Parkin ELISA Kit (JL11195, Shanghai Jianglai Industrial Limited by Share Ltd., Shanghai, China), Human PINK1 ELISA Kit (JL11175, Shanghai Jianglai Industrial Limited by Share Ltd.), Human/Rat FAS ELISA Kit (CSB-E04542h/CSB-E07324r, CUSABIO Co., Ltd.), Human/Rat ACC ELISA Kit (CSBEL001119HU, CUSABIO Co., Ltd./YJ059228, Shanghai Yuanju Biotechnology Center, Shanghai, China), and Human/Rat CD36 ELISA Kit (CSB-E10117h/CSBEL004927RA, CUSABIO Co., Ltd.).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: The Journal of international medical research

Article Title: The therapeutic potential of irisin in alleviating acute lung injury via inflammation and ferroptosis modulation.

doi: 10.1177/03000605251340338

Figure Lengend Snippet: Figure 6. Western blotting analysis on the effects of irisin on the expression level of ferroptosis-associated proteins. (a) Representative western blotting images for the expression of ACSL4, COX-2, GPX4, p-AMPK, and t-AMPK in lung tissues. GAPDH was selected as the loading control protein. (b) Quantification analysis of the related bands of ACSL4, COX-2, GPX4, p-AMPK, and t-AMPK in lung tissues. Lung tissues were harvested on day 3 post-CLP. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-hoc test. n ¼ 6 per group. Data are presented as means SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Subsequently, the membranes were incubated with rabbit anti-AMPK (MCE, Cat# HY-P80541), anti-p-AMPK (MCE, HYP80452), GPX4 (MCE, HY-P80450), ACSL4 (Santa Cruz Biotechnology, Dallas, Texas, USA; Cat# sc-365230), COX-2 (Abcam, Cambridge, United Kingdom; Cat# ab283574), and GAPDH (MCE, HY-P80137) antibodies at 4 C overnight.

Techniques: Western Blot, Expressing, Control

Journal: iScience

Article Title: Activation of AMP-activated protein kinase (AMPK) through inhibiting interaction with prohibitins

doi: 10.1016/j.isci.2023.106293

Figure Lengend Snippet:

Article Snippet: LANCE UltraEuropium-anti-phospho-Acetyl-CoA Carboxylase (Ser79) , PerkinElmer , Cat#TRF0208.

Techniques: Produced, FLAG-tag, Recombinant, Transfection, Silver Staining, Isolation, Cell Culture, Expressing, Plasmid Preparation, Luciferase, shRNA, Software